A low-cost paper-based platform for fast and reliable screening of cellular interactions with materials
: E. Rosqvist, E Niemelä, J. Frisk, H. Öblom, R. Koppolu, H. Abdelkader, D. Soto Véliz, M. Mennillo, A.P. Venu, P. Ihalainen, M. Aubert, N. Sandler, C.-E. Wilén, M. Toivakka, J.E. Eriksson, R. Österbacka, J. Peltonen,
Publisher: ROYAL SOC CHEMISTRY
: 2020
: Journal of Materials Chemistry B
: JOURNAL OF MATERIALS CHEMISTRY B
: J MATER CHEM B
: 8
: 6
: 1146
: 1156
: 11
: 2050-750X
: 2050-7518
DOI: https://doi.org/10.1039/c9tb01958h
: https://research.utu.fi/converis/portal/detail/Publication/46517596
A paper-based platform was developed and tested for studies on basic cell culture, material biocompatibility, and activity of pharmaceuticals in order to provide a reliable, robust and low-cost cell study platform. It is based upon a paper or paperboard support, with a nanostructured Latex coating to provide an enhanced cell growth and sufficient barrier properties. Wetting is Limited to regions of interest using a flexographically printed hydrophobic polydimethylsiloxane Layer with circular non-print areas. The nanostructured coating can be substituted for another coating of interest, or the regions of interest functionalized with a material to be studied. The platform is fully up-scalable, being produced with roll-to-roll rod coating, flexographic and inkjet printing methods. Results show that the platform efficiency is comparable to multi-well plates in colorimetric assays in three separate studies: a cell culture study, a biocompatibility study, and a drug screening study. The color intensity is quantified by using a common office scanner or an imaging device and the data is analyzed by a custom computer software without the need for expensive screening or analysis equipment.
UTU Research Portal