A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
A low-cost paper-based platform for fast and reliable screening of cellular interactions with materials
Tekijät: E. Rosqvist, E Niemelä, J. Frisk, H. Öblom, R. Koppolu, H. Abdelkader, D. Soto Véliz, M. Mennillo, A.P. Venu, P. Ihalainen, M. Aubert, N. Sandler, C.-E. Wilén, M. Toivakka, J.E. Eriksson, R. Österbacka, J. Peltonen,
Kustantaja: ROYAL SOC CHEMISTRY
Julkaisuvuosi: 2020
Journal: Journal of Materials Chemistry B
Tietokannassa oleva lehden nimi: JOURNAL OF MATERIALS CHEMISTRY B
Lehden akronyymi: J MATER CHEM B
Vuosikerta: 8
Numero: 6
Aloitussivu: 1146
Lopetussivu: 1156
Sivujen määrä: 11
ISSN: 2050-750X
eISSN: 2050-7518
DOI: https://doi.org/10.1039/c9tb01958h
Rinnakkaistallenteen osoite: https://research.utu.fi/converis/portal/detail/Publication/46517596
A paper-based platform was developed and tested for studies on basic cell culture, material biocompatibility, and activity of pharmaceuticals in order to provide a reliable, robust and low-cost cell study platform. It is based upon a paper or paperboard support, with a nanostructured Latex coating to provide an enhanced cell growth and sufficient barrier properties. Wetting is Limited to regions of interest using a flexographically printed hydrophobic polydimethylsiloxane Layer with circular non-print areas. The nanostructured coating can be substituted for another coating of interest, or the regions of interest functionalized with a material to be studied. The platform is fully up-scalable, being produced with roll-to-roll rod coating, flexographic and inkjet printing methods. Results show that the platform efficiency is comparable to multi-well plates in colorimetric assays in three separate studies: a cell culture study, a biocompatibility study, and a drug screening study. The color intensity is quantified by using a common office scanner or an imaging device and the data is analyzed by a custom computer software without the need for expensive screening or analysis equipment.
Ladattava julkaisu This is an electronic reprint of the original article. |  |
