Multikingdom oral microbiome interactions in early-onset cryptogenic ischemic stroke - UTU Research Portal

A1 Refereed original research article in a scientific journal

Multikingdom oral microbiome interactions in early-onset cryptogenic ischemic stroke

Authors: Manzoor, Muhammed; Leskelä, Jaakko; Pietiäinen, Milla; Martinez-Majander, Nicolas; Ylikotila, Pauli; Könönen, Eija; Niiranen, Teemu; Lahti, Leo; Sinisalo, Juha; Putaala, Jukka; Pussinen, Pirkko J; Paju, Susanna

Publisher: Springer Nature; International Society for Microbial Ecology

Publication year: 2024

Journal: ISME Communications

Journal name in source: ISME communications

Journal acronym: ISME Commun

Article number: ycae088

Volume: 4

Issue: 1

eISSN: 2730-6151

DOI: https://doi.org/10.1093/ismeco/ycae088

Web address : https://doi.org/10.1093/ismeco/ycae088

Self-archived copy’s web address: https://research.utu.fi/converis/portal/detail/Publication/457191176

Abstract

Although knowledge of the role of the oral microbiome in ischemic stroke is steadily increasing, little is known about the multikingdom microbiota interactions and their consequences. We enrolled participants from a prospective multicentre case-control study and investigated multikingdom microbiome differences using saliva metagenomic datasets (n = 308) from young patients diagnosed with cryptogenic ischemic stroke (CIS) and age- and sex-matched stroke-free controls. Differentially abundant taxa were identified using Analysis of Compositions of Microbiomes with Bias Correction (ANCOM-BC2). Functional potential was inferred using HUMANn3. Our findings revealed significant differences in the composition and functional capacity of the oral microbiota associated with CIS. We identified 51 microbial species, including 47 bacterial, 3 viral, and one fungal species associated with CIS in the adjusted model. Co-abundance network analysis highlighted a more intricate microbial network in CIS patients, indicating potential interactions and co-occurrence patterns among microbial species across kingdoms. The results of our metagenomic analysis reflect the complexity of the oral microbiome, with high diversity and multikingdom interactions, which may play a role in health and disease.

Downloadable publication

This is an electronic reprint of the original article.
This reprint may differ from the original in pagination and typographic detail. Please cite the original version.

ycae088.pdf

Funding information in the publication:
Open Access funding provided by University of Helsinki. This work was supported by the Academy of Finland grants (316777 and 355532 for S.P.; 340750 for P.J.P.), the Finnish Dental Society Apollonia (for P.J.P.), the Sigrid Jusélius Foundation (for P.J.P.), the Novo Nordisk Foundation (for P.J.P.) and the Minerva Foundation Selma and Maja-Lisa Selander’s Fund (for M.M.). The SECRETO study was funded by the Finnish Medical Foundation, the Academy of Finland (286246, 318075, 322656), the Helsinki and Uusimaa Hospital District (TYH2014407, TYH2018318), and the Sigrid Jusélius Foundation. The funders had no role in the design or execution of the study; the collection, management, analysis, or interpretation of data; the preparation, review, or approval of the manuscript; or the decision to submit the manuscript for publication.