Seventy-five EST-linked Atlantic salmon (Salmo salar L.) microsatellite markers and their cross-amplification in five salmonid species



: Vasemagi A, Nilsson J, Primmer CR

Publisher: BLACKWELL PUBLISHING

: 2005

: Molecular Ecology Notes

: MOLECULAR ECOLOGY NOTES

: MOL ECOL NOTES

: 5

: 2

: 282

: 288

: 7

: 1471-8278

DOI: https://doi.org/10.1111/j.1471-8286.2005.00902.x


An Atlantic salmon (Salmo salar L.) expressed sequence tag (EST) database consisting of 58 146 ESTs was screened for microsatellite sequences. Subsequent development of 75 polymorphic EST-associated microsatellite markers in this species is described together with cross-species amplification results of 133 gene-associated tandem repeat markers in five salmonid species (Salmo trutta, Oncorhynchus mykiss, Salvelinus aplinus, Thymallus thymallus, Coregonus lavaretus). The number of alleles among EST-linked microsatellites in Atlantic salmon ranged from two to 41 with an average of 12 alleles per locus. Cross-species amplification resulted in detection of a total of ill polymorphic locus-species combinations (12-32 loci per species).



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