A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Seventy-five EST-linked Atlantic salmon (Salmo salar L.) microsatellite markers and their cross-amplification in five salmonid species
Tekijät: Vasemagi A, Nilsson J, Primmer CR
Kustantaja: BLACKWELL PUBLISHING
Julkaisuvuosi: 2005
Journal: Molecular Ecology Notes
Tietokannassa oleva lehden nimi: MOLECULAR ECOLOGY NOTES
Lehden akronyymi: MOL ECOL NOTES
Vuosikerta: 5
Numero: 2
Aloitussivu: 282
Lopetussivu: 288
Sivujen määrä: 7
ISSN: 1471-8278
DOI: https://doi.org/10.1111/j.1471-8286.2005.00902.x
Tiivistelmä
An Atlantic salmon (Salmo salar L.) expressed sequence tag (EST) database consisting of 58 146 ESTs was screened for microsatellite sequences. Subsequent development of 75 polymorphic EST-associated microsatellite markers in this species is described together with cross-species amplification results of 133 gene-associated tandem repeat markers in five salmonid species (Salmo trutta, Oncorhynchus mykiss, Salvelinus aplinus, Thymallus thymallus, Coregonus lavaretus). The number of alleles among EST-linked microsatellites in Atlantic salmon ranged from two to 41 with an average of 12 alleles per locus. Cross-species amplification resulted in detection of a total of ill polymorphic locus-species combinations (12-32 loci per species).
An Atlantic salmon (Salmo salar L.) expressed sequence tag (EST) database consisting of 58 146 ESTs was screened for microsatellite sequences. Subsequent development of 75 polymorphic EST-associated microsatellite markers in this species is described together with cross-species amplification results of 133 gene-associated tandem repeat markers in five salmonid species (Salmo trutta, Oncorhynchus mykiss, Salvelinus aplinus, Thymallus thymallus, Coregonus lavaretus). The number of alleles among EST-linked microsatellites in Atlantic salmon ranged from two to 41 with an average of 12 alleles per locus. Cross-species amplification resulted in detection of a total of ill polymorphic locus-species combinations (12-32 loci per species).
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