A1 Refereed original research article in a scientific journal

Seventy-five EST-linked Atlantic salmon (Salmo salar L.) microsatellite markers and their cross-amplification in five salmonid species



Authors: Vasemagi A, Nilsson J, Primmer CR

Publisher: BLACKWELL PUBLISHING

Publication year: 2005

Journal: Molecular Ecology Notes

Journal name in source: MOLECULAR ECOLOGY NOTES

Journal acronym: MOL ECOL NOTES

Volume: 5

Issue: 2

First page : 282

Last page: 288

Number of pages: 7

ISSN: 1471-8278

DOI: https://doi.org/10.1111/j.1471-8286.2005.00902.x(external)


Abstract
An Atlantic salmon (Salmo salar L.) expressed sequence tag (EST) database consisting of 58 146 ESTs was screened for microsatellite sequences. Subsequent development of 75 polymorphic EST-associated microsatellite markers in this species is described together with cross-species amplification results of 133 gene-associated tandem repeat markers in five salmonid species (Salmo trutta, Oncorhynchus mykiss, Salvelinus aplinus, Thymallus thymallus, Coregonus lavaretus). The number of alleles among EST-linked microsatellites in Atlantic salmon ranged from two to 41 with an average of 12 alleles per locus. Cross-species amplification resulted in detection of a total of ill polymorphic locus-species combinations (12-32 loci per species).



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