Production and purification of recombinant human (alpha 2)C2 adrenergic receptor using Saccharomyces cerevisiae



: Kapat A, Jaakola VP, Heimo H, Liitti S, Heikinheimo P, Glumoff T, Goldman A

Publisher: KLUWER ACADEMIC PUBL

: 2000

Bioseparation

BIOSEPARATION

: BIOSEPARATION

: 9

: 3

: 167

: 172

: 6

: 0923-179X

DOI: https://doi.org/10.1023/A:1008150412294


The objective is to generate milligram quantities of recombinant human alpha(2)C2 adrenergic receptor for X-ray crystallographic studies. It has been cloned in Saccharomyces cerevisiae, and the production level is at best about 13 pmol/mg of membrane protein, as estimated by radio-ligand binding assay. The receptor is solubilized with sucrose monolaurate followed by immunoaffinity purification and reconstitution into phospholipid vesicles. The efficiency of solubilization and immuno-purification are 60% and 91%, respectively.



Last updated on 2025-13-10 at 14:06