A1 Refereed original research article in a scientific journal
Production and purification of recombinant human (alpha 2)C2 adrenergic receptor using Saccharomyces cerevisiae
Authors: Kapat A, Jaakola VP, Heimo H, Liitti S, Heikinheimo P, Glumoff T, Goldman A
Publisher: KLUWER ACADEMIC PUBL
Publication year: 2000
Journal:: Bioseparation
Journal name in source: BIOSEPARATION
Journal acronym: BIOSEPARATION
Volume: 9
Issue: 3
First page : 167
Last page: 172
Number of pages: 6
ISSN: 0923-179X
DOI: https://doi.org/10.1023/A:1008150412294
Abstract
The objective is to generate milligram quantities of recombinant human alpha(2)C2 adrenergic receptor for X-ray crystallographic studies. It has been cloned in Saccharomyces cerevisiae, and the production level is at best about 13 pmol/mg of membrane protein, as estimated by radio-ligand binding assay. The receptor is solubilized with sucrose monolaurate followed by immunoaffinity purification and reconstitution into phospholipid vesicles. The efficiency of solubilization and immuno-purification are 60% and 91%, respectively.
The objective is to generate milligram quantities of recombinant human alpha(2)C2 adrenergic receptor for X-ray crystallographic studies. It has been cloned in Saccharomyces cerevisiae, and the production level is at best about 13 pmol/mg of membrane protein, as estimated by radio-ligand binding assay. The receptor is solubilized with sucrose monolaurate followed by immunoaffinity purification and reconstitution into phospholipid vesicles. The efficiency of solubilization and immuno-purification are 60% and 91%, respectively.
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