A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Production and purification of recombinant human (alpha 2)C2 adrenergic receptor using Saccharomyces cerevisiae
Tekijät: Kapat A, Jaakola VP, Heimo H, Liitti S, Heikinheimo P, Glumoff T, Goldman A
Kustantaja: KLUWER ACADEMIC PUBL
Julkaisuvuosi: 2000
Lehti:: Bioseparation
Tietokannassa oleva lehden nimi: BIOSEPARATION
Lehden akronyymi: BIOSEPARATION
Vuosikerta: 9
Numero: 3
Aloitussivu: 167
Lopetussivu: 172
Sivujen määrä: 6
ISSN: 0923-179X
DOI: https://doi.org/10.1023/A:1008150412294
Tiivistelmä
The objective is to generate milligram quantities of recombinant human alpha(2)C2 adrenergic receptor for X-ray crystallographic studies. It has been cloned in Saccharomyces cerevisiae, and the production level is at best about 13 pmol/mg of membrane protein, as estimated by radio-ligand binding assay. The receptor is solubilized with sucrose monolaurate followed by immunoaffinity purification and reconstitution into phospholipid vesicles. The efficiency of solubilization and immuno-purification are 60% and 91%, respectively.
The objective is to generate milligram quantities of recombinant human alpha(2)C2 adrenergic receptor for X-ray crystallographic studies. It has been cloned in Saccharomyces cerevisiae, and the production level is at best about 13 pmol/mg of membrane protein, as estimated by radio-ligand binding assay. The receptor is solubilized with sucrose monolaurate followed by immunoaffinity purification and reconstitution into phospholipid vesicles. The efficiency of solubilization and immuno-purification are 60% and 91%, respectively.
Turun yliopiston Tutkimustietojärjestelmän portaali