Probe-level estimation improves the detection of differential splicing in Affymetrix exon array studies



: Laajala E, Aittokallio T, Lahesmaa R, Elo LL

Publisher: BIOMED CENTRAL LTD

: 2009

: Genome Biology

: GENOME BIOLOGY

: GENOME BIOL

: ARTN R77

: 10

: 7

: 12

: 1474-760X

DOI: https://doi.org/10.1186/gb-2009-10-7-r77

: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2728531/pdf/gb-2009-10-7-r77.pdf


The recent advent of exon microarrays has made it possible to reveal differences in alternative splicing events on a global scale. We introduce a novel statistical procedure that takes full advantage of the probe-level information on Affymetrix exon arrays when detecting differential splicing between sample groups. In comparison to existing ranking methods, the procedure shows superior reproducibility and accuracy in distinguishing true biological findings from background noise in high agreement with experimental validations.



Last updated on 2024-26-11 at 13:59