A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Probe-level estimation improves the detection of differential splicing in Affymetrix exon array studies
Tekijät: Laajala E, Aittokallio T, Lahesmaa R, Elo LL
Kustantaja: BIOMED CENTRAL LTD
Julkaisuvuosi: 2009
Journal: Genome Biology
Tietokannassa oleva lehden nimi: GENOME BIOLOGY
Lehden akronyymi: GENOME BIOL
Artikkelin numero: ARTN R77
Vuosikerta: 10
Numero: 7
Sivujen määrä: 12
ISSN: 1474-760X
DOI: https://doi.org/10.1186/gb-2009-10-7-r77
Verkko-osoite: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2728531/pdf/gb-2009-10-7-r77.pdf
Tiivistelmä
The recent advent of exon microarrays has made it possible to reveal differences in alternative splicing events on a global scale. We introduce a novel statistical procedure that takes full advantage of the probe-level information on Affymetrix exon arrays when detecting differential splicing between sample groups. In comparison to existing ranking methods, the procedure shows superior reproducibility and accuracy in distinguishing true biological findings from background noise in high agreement with experimental validations.
The recent advent of exon microarrays has made it possible to reveal differences in alternative splicing events on a global scale. We introduce a novel statistical procedure that takes full advantage of the probe-level information on Affymetrix exon arrays when detecting differential splicing between sample groups. In comparison to existing ranking methods, the procedure shows superior reproducibility and accuracy in distinguishing true biological findings from background noise in high agreement with experimental validations.
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