A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Impact of Dietary Polydextrose Fiber on the Human Gut Metabolome
Tekijät: Lamichhane S, Yde CC, Forssten S, Ouwehand AC, Saarinen M, Jensen HM, Gibson GR, Rastall R, Fava F, Bertram HC
Kustantaja: AMER CHEMICAL SOC
Julkaisuvuosi: 2014
Journal: Journal of Agricultural and Food Chemistry
Tietokannassa oleva lehden nimi: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Lehden akronyymi: J AGR FOOD CHEM
Vuosikerta: 62
Numero: 40
Aloitussivu: 9944
Lopetussivu: 9951
Sivujen määrä: 8
ISSN: 0021-8561
DOI: https://doi.org/10.1021/jf5031218
Verkko-osoite: https://pubs.acs.org/doi/abs/10.1021/jf5031218
Tiivistelmä
The aim of the present study was to elucidate the impact of polydextrose PDX an soluble fiber, on the human fecal metabolome by high-resolution nuclear magnetic resonance (NMR) spectroscopy-based metabolomics in a dietary intervention study (n = 12). Principal component analysis (PCA) revealed a strong effect of PDX consumption on the fecal metabolome, which could be mainly ascribed to the presence of undigested fiber and oligosaccharides formed from partial degradation of PDX. Our results demonstrate that NMR-based metabolomics is a useful technique for metabolite profiling of feces and for testing compliance to dietary fiber intake in such trials. In addition, novel associations between PDX and the levels of the fecal metabolites acetate and propionate could be identified. The establishment of a correlation between the fecal metabolome and levels of Bifidobacterium (R-2 = 0.66) and Bacteroides (R-2 = 0.46) demonstrates the potential of NMR-based metabolomics to elucidate metabolic activity of bacteria in the gut.
The aim of the present study was to elucidate the impact of polydextrose PDX an soluble fiber, on the human fecal metabolome by high-resolution nuclear magnetic resonance (NMR) spectroscopy-based metabolomics in a dietary intervention study (n = 12). Principal component analysis (PCA) revealed a strong effect of PDX consumption on the fecal metabolome, which could be mainly ascribed to the presence of undigested fiber and oligosaccharides formed from partial degradation of PDX. Our results demonstrate that NMR-based metabolomics is a useful technique for metabolite profiling of feces and for testing compliance to dietary fiber intake in such trials. In addition, novel associations between PDX and the levels of the fecal metabolites acetate and propionate could be identified. The establishment of a correlation between the fecal metabolome and levels of Bifidobacterium (R-2 = 0.66) and Bacteroides (R-2 = 0.46) demonstrates the potential of NMR-based metabolomics to elucidate metabolic activity of bacteria in the gut.
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