Synthesis and cellular uptake of fluorescently labeled multivalent hyaluronan disaccharide conjugates of oligonucleotides



: Karskela M, Virta P, Malinen M, Urtti A, Lonnberg H

: 2008

Collection Symposium Series

CHEMISTRY OF NUCLEIC ACID COMPONENTS

: COLL SYMPOS SERIES

: 10

: 369

: 371

: 3

: 978-80-86241-29-6


Clustered hyaluronan disaccharides were studied as mediators of cellular delivery of antisense oligonucleotides through receptor mediated endocytosis. A synthetic route for preparation of an appropriately protected hyaluronic acid dimer was devised. Three fluorescein-labeled phosphorothioate oligonucleotide glycoconjugates containing two, four or six hyaluronan disaccharides were prepared. The influence of the hyaluronan moieties on the cellular uptake of the thioated oligonucleotides was tested in a cell line expressing the hyaluronan receptor CD44.



Last updated on 2025-14-10 at 10:11