B1 Non-refereed article in a scientific journal
Synthesis and cellular uptake of fluorescently labeled multivalent hyaluronan disaccharide conjugates of oligonucleotides
Authors: Karskela M, Virta P, Malinen M, Urtti A, Lonnberg H
Publication year: 2008
Journal:: Collection Symposium Series
Journal name in source: CHEMISTRY OF NUCLEIC ACID COMPONENTS
Journal acronym: COLL SYMPOS SERIES
Volume: 10
First page : 369
Last page: 371
Number of pages: 3
ISBN: 978-80-86241-29-6
Abstract
Clustered hyaluronan disaccharides were studied as mediators of cellular delivery of antisense oligonucleotides through receptor mediated endocytosis. A synthetic route for preparation of an appropriately protected hyaluronic acid dimer was devised. Three fluorescein-labeled phosphorothioate oligonucleotide glycoconjugates containing two, four or six hyaluronan disaccharides were prepared. The influence of the hyaluronan moieties on the cellular uptake of the thioated oligonucleotides was tested in a cell line expressing the hyaluronan receptor CD44.
Clustered hyaluronan disaccharides were studied as mediators of cellular delivery of antisense oligonucleotides through receptor mediated endocytosis. A synthetic route for preparation of an appropriately protected hyaluronic acid dimer was devised. Three fluorescein-labeled phosphorothioate oligonucleotide glycoconjugates containing two, four or six hyaluronan disaccharides were prepared. The influence of the hyaluronan moieties on the cellular uptake of the thioated oligonucleotides was tested in a cell line expressing the hyaluronan receptor CD44.
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