B1 Vertaisarvioimaton kirjoitus tieteellisessä lehdessä
Synthesis and cellular uptake of fluorescently labeled multivalent hyaluronan disaccharide conjugates of oligonucleotides
Tekijät: Karskela M, Virta P, Malinen M, Urtti A, Lonnberg H
Julkaisuvuosi: 2008
Lehti:: Collection Symposium Series
Tietokannassa oleva lehden nimi: CHEMISTRY OF NUCLEIC ACID COMPONENTS
Lehden akronyymi: COLL SYMPOS SERIES
Vuosikerta: 10
Aloitussivu: 369
Lopetussivu: 371
Sivujen määrä: 3
ISBN: 978-80-86241-29-6
Tiivistelmä
Clustered hyaluronan disaccharides were studied as mediators of cellular delivery of antisense oligonucleotides through receptor mediated endocytosis. A synthetic route for preparation of an appropriately protected hyaluronic acid dimer was devised. Three fluorescein-labeled phosphorothioate oligonucleotide glycoconjugates containing two, four or six hyaluronan disaccharides were prepared. The influence of the hyaluronan moieties on the cellular uptake of the thioated oligonucleotides was tested in a cell line expressing the hyaluronan receptor CD44.
Clustered hyaluronan disaccharides were studied as mediators of cellular delivery of antisense oligonucleotides through receptor mediated endocytosis. A synthetic route for preparation of an appropriately protected hyaluronic acid dimer was devised. Three fluorescein-labeled phosphorothioate oligonucleotide glycoconjugates containing two, four or six hyaluronan disaccharides were prepared. The influence of the hyaluronan moieties on the cellular uptake of the thioated oligonucleotides was tested in a cell line expressing the hyaluronan receptor CD44.
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