SYNTHESIS AND PRIMER PROPERTIES OF OLIGONUCLEOTIDES CONTAINING 3'-DEOXYPSICOTHYMIDINE UNITS, LABELED WITH FLUORESCEIN AT THE 1'-POSITION

: GUZAEV A, AZHAYEVA E, HOVINEN J, AZHAYEV A, LONNBERG H

Publisher: AMER CHEMICAL SOC

: 1994

Bioconjugate Chemistry

BIOCONJUGATE CHEMISTRY

: BIOCONJUGATE CHEM

: 5

: 6

: 501

: 503

: 3

: 1043-1802

DOI: https://doi.org/10.1021/bc00030a003

Several analogues of the standard M13 sequencing primer that contain up to five 3'-deoxypsicothymidines, or one or two such units labeled with fluorescein at the 1'-position, have been prepared. All these oligonucleotides have been shown to prime the DNA-polymerase-catalyzed synthesis of DNA.

Chemical sciences