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SYNTHESIS AND PRIMER PROPERTIES OF OLIGONUCLEOTIDES CONTAINING 3'-DEOXYPSICOTHYMIDINE UNITS, LABELED WITH FLUORESCEIN AT THE 1'-POSITION
Tekijät: GUZAEV A, AZHAYEVA E, HOVINEN J, AZHAYEV A, LONNBERG H
Kustantaja: AMER CHEMICAL SOC
Julkaisuvuosi: 1994
Lehti:: Bioconjugate Chemistry
Tietokannassa oleva lehden nimi: BIOCONJUGATE CHEMISTRY
Lehden akronyymi: BIOCONJUGATE CHEM
Vuosikerta: 5
Numero: 6
Aloitussivu: 501
Lopetussivu: 503
Sivujen määrä: 3
ISSN: 1043-1802
DOI: https://doi.org/10.1021/bc00030a003
Tiivistelmä
Several analogues of the standard M13 sequencing primer that contain up to five 3'-deoxypsicothymidines, or one or two such units labeled with fluorescein at the 1'-position, have been prepared. All these oligonucleotides have been shown to prime the DNA-polymerase-catalyzed synthesis of DNA.
Several analogues of the standard M13 sequencing primer that contain up to five 3'-deoxypsicothymidines, or one or two such units labeled with fluorescein at the 1'-position, have been prepared. All these oligonucleotides have been shown to prime the DNA-polymerase-catalyzed synthesis of DNA.