A1 Refereed original research article in a scientific journal

SYNTHESIS AND PRIMER PROPERTIES OF OLIGONUCLEOTIDES CONTAINING 3'-DEOXYPSICOTHYMIDINE UNITS, LABELED WITH FLUORESCEIN AT THE 1'-POSITION



Authors: GUZAEV A, AZHAYEVA E, HOVINEN J, AZHAYEV A, LONNBERG H

Publisher: AMER CHEMICAL SOC

Publication year: 1994

Journal:: Bioconjugate Chemistry

Journal name in source: BIOCONJUGATE CHEMISTRY

Journal acronym: BIOCONJUGATE CHEM

Volume: 5

Issue: 6

First page : 501

Last page: 503

Number of pages: 3

ISSN: 1043-1802

DOI: https://doi.org/10.1021/bc00030a003


Abstract
Several analogues of the standard M13 sequencing primer that contain up to five 3'-deoxypsicothymidines, or one or two such units labeled with fluorescein at the 1'-position, have been prepared. All these oligonucleotides have been shown to prime the DNA-polymerase-catalyzed synthesis of DNA.


Research Areas



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