A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Expression, purification and crystallization of a family 55 [beta]-1,3-glucanase from Chaetomium thermophilum
Tekijät: Papageorgiou AC, Li D
Kustantaja: International Union of Crystallography
Julkaisuvuosi: 2015
Journal: Acta Crystallographica Section F: Structural Biology Communications
Tietokannassa oleva lehden nimi: Acta Crystallographica Section F
Lehden akronyymi: Acta Cryst. F
Vuosikerta: 71
Numero: 6
Aloitussivu: 680
Lopetussivu: 683
Sivujen määrä: 4
ISSN: 2053-230X
DOI: https://doi.org/10.1107/S2053230X15006366
Verkko-osoite: http://dx.doi.org/10.1107/S2053230X15006366
A [beta]-1,3-glucanase from the thermophilic fungus Chaetomium thermophilum was overexpressed in Pichia pastoris, purified and crystallized in the presence of 1.8 M sodium/potassium phosphate pH 6.8 as a precipitant. Data to 2.0 A resolution were collected in-house at 293 K from a single crystal. The crystal was found to belong to space group P21, with unit-cell parameters a = 64.1, b = 85.8, c = 68.5 A, [beta] = 93.1degrees and one molecule in the asymmetric unit.
