A1 Refereed original research article in a scientific journal
Expression, purification and crystallization of a family 55 [beta]-1,3-glucanase from Chaetomium thermophilum
Authors: Papageorgiou AC, Li D
Publisher: International Union of Crystallography
Publication year: 2015
Journal: Acta Crystallographica Section F: Structural Biology Communications
Journal name in source: Acta Crystallographica Section F
Journal acronym: Acta Cryst. F
Volume: 71
Issue: 6
First page : 680
Last page: 683
Number of pages: 4
ISSN: 2053-230X
DOI: https://doi.org/10.1107/S2053230X15006366
Web address : http://dx.doi.org/10.1107/S2053230X15006366
A [beta]-1,3-glucanase from the thermophilic fungus Chaetomium thermophilum was overexpressed in Pichia pastoris, purified and crystallized in the presence of 1.8 M sodium/potassium phosphate pH 6.8 as a precipitant. Data to 2.0 A resolution were collected in-house at 293 K from a single crystal. The crystal was found to belong to space group P21, with unit-cell parameters a = 64.1, b = 85.8, c = 68.5 A, [beta] = 93.1degrees and one molecule in the asymmetric unit.
