The Cu2+-promoted cleavage of mRNA 5 '-cap analogs: A kinetic study with P-1-(7-methylguanosin-5 '-yl) P-3-(nucleosid-5 '-yl) triphospates and P-1-(7-methylguanosin-5 '-yl) P-4-(guanosin-5 '-yl) tetraphosphate

: Wieczorek Z, Darzynkiewicz E, Kuusela S, Lonnberg H

Publisher: MARCEL DEKKER INC

: 1999

: Nucleosides & nucleotides

: NUCLEOSIDES & NUCLEOTIDES

: NUCLEOS NUCLEOT

: 18

: 1

: 11

: 21

: 11

: 0732-8311

DOI: https://doi.org/10.1080/07328319908045590

A kinetic study on the cleavage of a number of mRNA 5'-cap analogs, m(7)GpppN and m(7)GppppG, with Cu2+ aquo ion has been performed. Time-dependent product distributions at various pH and metal ion concentrations have been determined by capillary zone electrophoresis, and these data have been used to calculate the rate constants for various parallel reactions of the breakdown of the cap analogs.

Chemical sciences