Two-photon excitation fluorescence microscopy using a semiconductor laser

: Hanninen P., Schrader M., Soini E., Hell S.

: 1995

Bioimaging

: Bioimaging

: 3

: 2

: 70

: 75

: 6

: 0966-9051

DOI: https://doi.org/10.1002/1361-6374(199506)3:2<70::AID-BIO3>3.0.CO;2-W

: http://api.elsevier.com/content/abstract/scopus_id:0029145831

Parameters for two-photon microscopy using continuous wave light excitation are evaluated. Continuous wave two-photon excitation is compared with pulsed pico- and sub-picosecond two-photon excitation with fluorescent test samples and biological material. Furthermore, we show the first two-photon excitation fluorescent images using a semiconductor laser as a light source.