A1 Refereed original research article in a scientific journal

Two-photon excitation fluorescence microscopy using a semiconductor laser



Authors: Hanninen P., Schrader M., Soini E., Hell S.

Publication year: 1995

Journal:: Bioimaging

Journal name in source: Bioimaging

Volume: 3

Issue: 2

First page : 70

Last page: 75

Number of pages: 6

ISSN: 0966-9051

DOI: https://doi.org/10.1002/1361-6374(199506)3:2<70::AID-BIO3>3.0.CO;2-W

Web address : http://api.elsevier.com/content/abstract/scopus_id:0029145831


Abstract
Parameters for two-photon microscopy using continuous wave light excitation are evaluated. Continuous wave two-photon excitation is compared with pulsed pico- and sub-picosecond two-photon excitation with fluorescent test samples and biological material. Furthermore, we show the first two-photon excitation fluorescent images using a semiconductor laser as a light source.



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