A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Ultrasonic enrichment of microspheres for ultrasensitive biomedical analysis in confocal laser-scanning fluorescence detection
Tekijät: Wiklund M., Toivonen J., Tirri M., Hänninen P., Hertz H.
Julkaisuvuosi: 2004
Lehti:: Journal of Applied Physics
Tietokannassa oleva lehden nimi: Journal of Applied Physics
Vuosikerta: 96
Numero: 2
Aloitussivu: 1242
Lopetussivu: 1248
Sivujen määrä: 7
ISSN: 0021-8979
DOI: https://doi.org/10.1063/1.1763226
Verkko-osoite: http://api.elsevier.com/content/abstract/scopus_id:3242667289
Tiivistelmä
Ultrasensitive biomedical analysis by concentration of biologically active microspheres was discussed. For the study, an ultrasonic particle concentrator (UPC) based on a standing-wave hemispherical resonator was combined with a confocal-laser scanning fluorescence detection. The UPC collects microspheres in plane, dense, single-layer aggregates at well-defined positions controlled by the acoustic resonator geometry and the acoustic frequency, far from the vessel boundary. It is suggested that two major factors that influence the sensitivity of a single-step homogeneous microsphere-based assay are the analyte-to-microsphere ratio and the concentration of microspheres in the sample.
Ultrasensitive biomedical analysis by concentration of biologically active microspheres was discussed. For the study, an ultrasonic particle concentrator (UPC) based on a standing-wave hemispherical resonator was combined with a confocal-laser scanning fluorescence detection. The UPC collects microspheres in plane, dense, single-layer aggregates at well-defined positions controlled by the acoustic resonator geometry and the acoustic frequency, far from the vessel boundary. It is suggested that two major factors that influence the sensitivity of a single-step homogeneous microsphere-based assay are the analyte-to-microsphere ratio and the concentration of microspheres in the sample.
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