A1 Refereed original research article in a scientific journal
Ultrasonic enrichment of microspheres for ultrasensitive biomedical analysis in confocal laser-scanning fluorescence detection
Authors: Wiklund M., Toivonen J., Tirri M., Hänninen P., Hertz H.
Publication year: 2004
Journal:: Journal of Applied Physics
Journal name in source: Journal of Applied Physics
Volume: 96
Issue: 2
First page : 1242
Last page: 1248
Number of pages: 7
ISSN: 0021-8979
DOI: https://doi.org/10.1063/1.1763226
Web address : http://api.elsevier.com/content/abstract/scopus_id:3242667289
Abstract
Ultrasensitive biomedical analysis by concentration of biologically active microspheres was discussed. For the study, an ultrasonic particle concentrator (UPC) based on a standing-wave hemispherical resonator was combined with a confocal-laser scanning fluorescence detection. The UPC collects microspheres in plane, dense, single-layer aggregates at well-defined positions controlled by the acoustic resonator geometry and the acoustic frequency, far from the vessel boundary. It is suggested that two major factors that influence the sensitivity of a single-step homogeneous microsphere-based assay are the analyte-to-microsphere ratio and the concentration of microspheres in the sample.
Ultrasensitive biomedical analysis by concentration of biologically active microspheres was discussed. For the study, an ultrasonic particle concentrator (UPC) based on a standing-wave hemispherical resonator was combined with a confocal-laser scanning fluorescence detection. The UPC collects microspheres in plane, dense, single-layer aggregates at well-defined positions controlled by the acoustic resonator geometry and the acoustic frequency, far from the vessel boundary. It is suggested that two major factors that influence the sensitivity of a single-step homogeneous microsphere-based assay are the analyte-to-microsphere ratio and the concentration of microspheres in the sample.
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