Synthesis of phosphate-branched oligonucleotides

: Heinonen P, Lonnberg H

Publisher: AMER CHEMICAL SOC

: 2004

: Bioconjugate Chemistry

: BIOCONJUGATE CHEMISTRY

: BIOCONJUGATE CHEM

: 15

: 6

: 1158

: 1160

: 3

: 1043-1802

DOI: https://doi.org/10.1021/bc049845g

A solid-phase synthesis for phosphate-branched oligonucleotides is described. The method is based on coupling of a single nucleoside phosphorodiamidite to terminal hydroxyl functions of two solid-supported oligonucleotides. After oxidation of the phosphite triester obtained to a phosphate triester, the third branch is assembled by conventional phosphoramidite chemistry.

Chemical sciences