Oligonucleotides Incorporating Palladacyclic Nucleobase Surrogates



: Maity SK, Lonnberg T

Publisher: WILEY-V C H VERLAG GMBH

: 2018

: Chemistry - A European Journal

: CHEMISTRY-A EUROPEAN JOURNAL

: CHEM-EUR J

: 24

: 6

: 1274

: 1277

: 4

: 0947-6539

DOI: https://doi.org/10.1002/chem.201705797

: https://research.utu.fi/converis/portal/detail/Publication/30491162


An oligonucleotide incorporating a palladacyclic nucleobase has been prepared by ligand-directed metalation of a phenylpyridine moiety. This oligonucleotide hybridized with natural counterparts placing any of the canonical nucleobases opposite to the palladacyclic residue. The palladated duplexes had B-type conformation and melting temperatures comparable to those of respective unmodified duplexes with a single mismatch. In the duplexes placing C, G or T (but not A) opposite to the palladacyclic residue, greatly increased absorptivity suggested formation of a Pd-II-mediated base pair. Absorptivity and ellipticity of these duplexes persisted even at the highest temperatures applicable in T-m and CD experiments (90 degrees C). Evidently the Pd-II-mediated base pairs do not dissociate under the experimental conditions.

Last updated on 2024-26-11 at 15:08