A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Oligonucleotides Incorporating Palladacyclic Nucleobase Surrogates
Tekijät: Maity SK, Lonnberg T
Kustantaja: WILEY-V C H VERLAG GMBH
Julkaisuvuosi: 2018
Journal: Chemistry - A European Journal
Tietokannassa oleva lehden nimi: CHEMISTRY-A EUROPEAN JOURNAL
Lehden akronyymi: CHEM-EUR J
Vuosikerta: 24
Numero: 6
Aloitussivu: 1274
Lopetussivu: 1277
Sivujen määrä: 4
ISSN: 0947-6539
DOI: https://doi.org/10.1002/chem.201705797
Rinnakkaistallenteen osoite: https://research.utu.fi/converis/portal/detail/Publication/30491162
Tiivistelmä
An oligonucleotide incorporating a palladacyclic nucleobase has been prepared by ligand-directed metalation of a phenylpyridine moiety. This oligonucleotide hybridized with natural counterparts placing any of the canonical nucleobases opposite to the palladacyclic residue. The palladated duplexes had B-type conformation and melting temperatures comparable to those of respective unmodified duplexes with a single mismatch. In the duplexes placing C, G or T (but not A) opposite to the palladacyclic residue, greatly increased absorptivity suggested formation of a Pd-II-mediated base pair. Absorptivity and ellipticity of these duplexes persisted even at the highest temperatures applicable in T-m and CD experiments (90 degrees C). Evidently the Pd-II-mediated base pairs do not dissociate under the experimental conditions.
An oligonucleotide incorporating a palladacyclic nucleobase has been prepared by ligand-directed metalation of a phenylpyridine moiety. This oligonucleotide hybridized with natural counterparts placing any of the canonical nucleobases opposite to the palladacyclic residue. The palladated duplexes had B-type conformation and melting temperatures comparable to those of respective unmodified duplexes with a single mismatch. In the duplexes placing C, G or T (but not A) opposite to the palladacyclic residue, greatly increased absorptivity suggested formation of a Pd-II-mediated base pair. Absorptivity and ellipticity of these duplexes persisted even at the highest temperatures applicable in T-m and CD experiments (90 degrees C). Evidently the Pd-II-mediated base pairs do not dissociate under the experimental conditions.
Ladattava julkaisu This is an electronic reprint of the original article. |  |
Turun yliopiston Tutkimustietojärjestelmän portaali