From research to routines - Ultrafast laser systems can be replaced by low cost lasers in two-photon excitation applications



: Tirri M., Vaarno J., Soini J., Hänninen P.

: Boccara A.-C.

: 2003

: Proceedings of SPIE - The International Society for Optical Engineering

: 5143

: 193

: 201

: 9

: http://api.elsevier.com/content/abstract/scopus_id:1342309736


This paper outlines the latest developments in two-photon excitation laboratory instrumentation using low-cost laser equipment. Our research group has shown that application of two-photon excitation need not to be limited to those laboratories that can afford the price and maintenance of ultrafast laser systems. With certain compromises and well-designed experiments, low-cost lasers, such as the Q-switched Nd:YAG microchip laser, can be used in utilizing the positive properties of two-photon excitation. Results using microparticles or cells as bioactive carriers confirm this fact. Application examples from biomedical research as well from clinical environment are presented.



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