A3 Vertaisarvioitu kirjan tai muun kokoomateoksen osa
Label-Free Quantitation for Clinical Proteomics
Tekijät: Robert Moulder, Young Ah Goo, David R. Goodlett
Toimittaja: Salvatore Sechi
Kustannuspaikka: New York
Julkaisuvuosi: 2016
Kokoomateoksen nimi: Quantitative Proteomics by Mass Spectrometry
Sarjan nimi: Methods in Molecular Biology
Vuosikerta: 1410
Aloitussivu: 65
Lopetussivu: 76
Sivujen määrä: 12
ISBN: 978-1-4939-3522-2
eISBN: 978-1-4939-3524-6
ISSN: 1064-3745
DOI: https://doi.org/10.1007/978-1-4939-3524-6_4
Verkko-osoite: http://link.springer.com/protocol/10.1007/978-1-4939-3524-6_4
Label free quantification (LFQ) has emerged as viable option for quantitative LC-MS/MS-based proteomic analyses for use on the scale of hundreds of samples such as are encountered in clinical analysis. Notably, sample preparation, sample loading, HPLC separations and mass spectrometric performance must be highly reproducible for this approach to be effective. The following protocols describe the key steps in the methods related to sample preparation and analysis for LC-MS/MS based label-free quantitation using standard data-dependent acquisition.
Ladattava julkaisu This is an electronic reprint of the original article. |  |
