A3 Refereed book chapter or chapter in a compilation book
Label-Free Quantitation for Clinical Proteomics
Authors: Robert Moulder, Young Ah Goo, David R. Goodlett
Editors: Salvatore Sechi
Publishing place: New York
Publication year: 2016
Book title : Quantitative Proteomics by Mass Spectrometry
Series title: Methods in Molecular Biology
Volume: 1410
First page : 65
Last page: 76
Number of pages: 12
ISBN: 978-1-4939-3522-2
eISBN: 978-1-4939-3524-6
ISSN: 1064-3745
DOI: https://doi.org/10.1007/978-1-4939-3524-6_4
Web address : http://link.springer.com/protocol/10.1007/978-1-4939-3524-6_4
Label free quantification (LFQ) has emerged as viable option for quantitative LC-MS/MS-based proteomic analyses for use on the scale of hundreds of samples such as are encountered in clinical analysis. Notably, sample preparation, sample loading, HPLC separations and mass spectrometric performance must be highly reproducible for this approach to be effective. The following protocols describe the key steps in the methods related to sample preparation and analysis for LC-MS/MS based label-free quantitation using standard data-dependent acquisition.
Downloadable publication This is an electronic reprint of the original article. |  |
