A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Fluorescent Oligonucleotide Probes for Screening High-Affinity Nucleobase Surrogates
Tekijät: Aro-Heinila A, Lönnberg T
Kustantaja: WILEY-V C H VERLAG GMBH
Julkaisuvuosi: 2017
Journal: Chemistry - A European Journal
Tietokannassa oleva lehden nimi: CHEMISTRY-A EUROPEAN JOURNAL
Lehden akronyymi: CHEM-EUR J
Vuosikerta: 23
Numero: 5
Aloitussivu: 1028
Lopetussivu: 1031
Sivujen määrä: 4
ISSN: 0947-6539
eISSN: 1521-3765
DOI: https://doi.org/10.1002/chem.201605300
Rinnakkaistallenteen osoite: https://research.utu.fi/converis/portal/detail/Publication/28146226
Double-helical oligonucleotide probes featuring a single-nucleotide gap opposed by one of the canonical nucleobases and flanked by the fluorescent nucleobase analogue pyrrolocytosine have been synthesized and titrated with PdII chelates of dipicolinamide and its N-2, N-6-di-alkylated derivatives. The fluorometric titrations revealed greatly increased affinity of the PdII chelates for the nucleobases opposing the gap compared to the respective free nucleotides in solution. Owing to the constrained environment of the single-nucleotide gap, the relative stabilities of the various PdII-mediated base pairs were also significantly different from those previously reported at monomer level.
Ladattava julkaisu This is an electronic reprint of the original article. |  |
