A1 Refereed original research article in a scientific journal
Fluorescent Oligonucleotide Probes for Screening High-Affinity Nucleobase Surrogates
Authors: Aro-Heinila A, Lönnberg T
Publisher: WILEY-V C H VERLAG GMBH
Publication year: 2017
Journal: Chemistry - A European Journal
Journal name in source: CHEMISTRY-A EUROPEAN JOURNAL
Journal acronym: CHEM-EUR J
Volume: 23
Issue: 5
First page : 1028
Last page: 1031
Number of pages: 4
ISSN: 0947-6539
eISSN: 1521-3765
DOI: https://doi.org/10.1002/chem.201605300
Self-archived copy’s web address: https://research.utu.fi/converis/portal/detail/Publication/28146226
Double-helical oligonucleotide probes featuring a single-nucleotide gap opposed by one of the canonical nucleobases and flanked by the fluorescent nucleobase analogue pyrrolocytosine have been synthesized and titrated with PdII chelates of dipicolinamide and its N-2, N-6-di-alkylated derivatives. The fluorometric titrations revealed greatly increased affinity of the PdII chelates for the nucleobases opposing the gap compared to the respective free nucleotides in solution. Owing to the constrained environment of the single-nucleotide gap, the relative stabilities of the various PdII-mediated base pairs were also significantly different from those previously reported at monomer level.
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