Analysis of F-18-labelled synthesis products on TLC plates: Comparison of radioactivity scanning, film auto radiography, and a phosphoimaging technique



: Kamarainen EL, Haaparanta M, Siitari-Kauppi M, Koivula T, Lipponen T, Solin O

Publisher: PERGAMON-ELSEVIER SCIENCE LTD

: 2006

: Applied Radiation and Isotopes

: APPLIED RADIATION AND ISOTOPES

: APPL RADIAT ISOTOPES

: 64

: 9

: 1043

: 1047

: 5

: 0969-8043

DOI: https://doi.org/10.1016/j.apradiso.2006.04.012


We compared radioactivity scanning, film autoradiography, and digital photostimulated luminescence (PSL) autoradiography (phosphoimaging technique) in detection of radioactivity on thin-layer chromatography (TLC) plates. TLC combined with radioactivity detection is rapid, simple, and relatively flexible. Here, F-18-labelled synthesis products were analyzed by TLC and the radioactivity distribution on the plates determined using the three techniques. Radioactivity scanning is appropriate only with good chromatographic resolution and previously validated scanning parameters. Film autoradiography exhibits poor linearity if radioactivity varies greatly. PSL provides high sensitivity and resolution and superior linearity compared with the other methods. (c) 2006 Elsevier Ltd. All rights reserved.



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