A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Analysis of F-18-labelled synthesis products on TLC plates: Comparison of radioactivity scanning, film auto radiography, and a phosphoimaging technique
Tekijät: Kamarainen EL, Haaparanta M, Siitari-Kauppi M, Koivula T, Lipponen T, Solin O
Kustantaja: PERGAMON-ELSEVIER SCIENCE LTD
Julkaisuvuosi: 2006
Journal: Applied Radiation and Isotopes
Tietokannassa oleva lehden nimi: APPLIED RADIATION AND ISOTOPES
Lehden akronyymi: APPL RADIAT ISOTOPES
Vuosikerta: 64
Numero: 9
Aloitussivu: 1043
Lopetussivu: 1047
Sivujen määrä: 5
ISSN: 0969-8043
DOI: https://doi.org/10.1016/j.apradiso.2006.04.012
Tiivistelmä
We compared radioactivity scanning, film autoradiography, and digital photostimulated luminescence (PSL) autoradiography (phosphoimaging technique) in detection of radioactivity on thin-layer chromatography (TLC) plates. TLC combined with radioactivity detection is rapid, simple, and relatively flexible. Here, F-18-labelled synthesis products were analyzed by TLC and the radioactivity distribution on the plates determined using the three techniques. Radioactivity scanning is appropriate only with good chromatographic resolution and previously validated scanning parameters. Film autoradiography exhibits poor linearity if radioactivity varies greatly. PSL provides high sensitivity and resolution and superior linearity compared with the other methods. (c) 2006 Elsevier Ltd. All rights reserved.
We compared radioactivity scanning, film autoradiography, and digital photostimulated luminescence (PSL) autoradiography (phosphoimaging technique) in detection of radioactivity on thin-layer chromatography (TLC) plates. TLC combined with radioactivity detection is rapid, simple, and relatively flexible. Here, F-18-labelled synthesis products were analyzed by TLC and the radioactivity distribution on the plates determined using the three techniques. Radioactivity scanning is appropriate only with good chromatographic resolution and previously validated scanning parameters. Film autoradiography exhibits poor linearity if radioactivity varies greatly. PSL provides high sensitivity and resolution and superior linearity compared with the other methods. (c) 2006 Elsevier Ltd. All rights reserved.
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