A1 Refereed original research article in a scientific journal
Analysis of F-18-labelled synthesis products on TLC plates: Comparison of radioactivity scanning, film auto radiography, and a phosphoimaging technique
Authors: Kamarainen EL, Haaparanta M, Siitari-Kauppi M, Koivula T, Lipponen T, Solin O
Publisher: PERGAMON-ELSEVIER SCIENCE LTD
Publication year: 2006
Journal: Applied Radiation and Isotopes
Journal name in source: APPLIED RADIATION AND ISOTOPES
Journal acronym: APPL RADIAT ISOTOPES
Volume: 64
Issue: 9
First page : 1043
Last page: 1047
Number of pages: 5
ISSN: 0969-8043
DOI: https://doi.org/10.1016/j.apradiso.2006.04.012
Abstract
We compared radioactivity scanning, film autoradiography, and digital photostimulated luminescence (PSL) autoradiography (phosphoimaging technique) in detection of radioactivity on thin-layer chromatography (TLC) plates. TLC combined with radioactivity detection is rapid, simple, and relatively flexible. Here, F-18-labelled synthesis products were analyzed by TLC and the radioactivity distribution on the plates determined using the three techniques. Radioactivity scanning is appropriate only with good chromatographic resolution and previously validated scanning parameters. Film autoradiography exhibits poor linearity if radioactivity varies greatly. PSL provides high sensitivity and resolution and superior linearity compared with the other methods. (c) 2006 Elsevier Ltd. All rights reserved.
We compared radioactivity scanning, film autoradiography, and digital photostimulated luminescence (PSL) autoradiography (phosphoimaging technique) in detection of radioactivity on thin-layer chromatography (TLC) plates. TLC combined with radioactivity detection is rapid, simple, and relatively flexible. Here, F-18-labelled synthesis products were analyzed by TLC and the radioactivity distribution on the plates determined using the three techniques. Radioactivity scanning is appropriate only with good chromatographic resolution and previously validated scanning parameters. Film autoradiography exhibits poor linearity if radioactivity varies greatly. PSL provides high sensitivity and resolution and superior linearity compared with the other methods. (c) 2006 Elsevier Ltd. All rights reserved.
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