A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Cost-effective genome-wide estimation of allele frequencies from pooled DNA in Atlantic salmon (Salmo salar L.)
Tekijät: Ozerov M, Vasemägi A, Wennevik V, Niemelä E, Prusov S, Kent M, Vähä JP
Kustantaja: BIOMED CENTRAL LTD
Julkaisuvuosi: 2013
Journal: BMC Genomics
Tietokannassa oleva lehden nimi: BMC GENOMICS
Lehden akronyymi: BMC GENOMICS
Artikkelin numero: ARTN 12
Vuosikerta: 14
Sivujen määrä: 9
ISSN: 1471-2164
DOI: https://doi.org/10.1186/1471-2164-14-12
Verkko-osoite: http://www.biomedcentral.com/1471-2164/14/12
Tiivistelmä
Conclusions: Despite of higher level of variation associated with array replicates compared to pool construction, we suggest that both sources of variation should be taken into account. This study demonstrates that DNA pooling allows fast and high-throughput determination of allele frequencies in Atlantic salmon enabling cost-efficient identification of informative markers for discrimination of populations at various geographical scales, as well as identification of loci controlling ecologically and economically important traits.
Conclusions: Despite of higher level of variation associated with array replicates compared to pool construction, we suggest that both sources of variation should be taken into account. This study demonstrates that DNA pooling allows fast and high-throughput determination of allele frequencies in Atlantic salmon enabling cost-efficient identification of informative markers for discrimination of populations at various geographical scales, as well as identification of loci controlling ecologically and economically important traits.
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