A1 Refereed original research article in a scientific journal
Cost-effective genome-wide estimation of allele frequencies from pooled DNA in Atlantic salmon (Salmo salar L.)
Authors: Ozerov M, Vasemägi A, Wennevik V, Niemelä E, Prusov S, Kent M, Vähä JP
Publisher: BIOMED CENTRAL LTD
Publication year: 2013
Journal: BMC Genomics
Journal name in source: BMC GENOMICS
Journal acronym: BMC GENOMICS
Article number: ARTN 12
Volume: 14
Number of pages: 9
ISSN: 1471-2164
DOI: https://doi.org/10.1186/1471-2164-14-12
Web address : http://www.biomedcentral.com/1471-2164/14/12
Abstract
Conclusions: Despite of higher level of variation associated with array replicates compared to pool construction, we suggest that both sources of variation should be taken into account. This study demonstrates that DNA pooling allows fast and high-throughput determination of allele frequencies in Atlantic salmon enabling cost-efficient identification of informative markers for discrimination of populations at various geographical scales, as well as identification of loci controlling ecologically and economically important traits.
Conclusions: Despite of higher level of variation associated with array replicates compared to pool construction, we suggest that both sources of variation should be taken into account. This study demonstrates that DNA pooling allows fast and high-throughput determination of allele frequencies in Atlantic salmon enabling cost-efficient identification of informative markers for discrimination of populations at various geographical scales, as well as identification of loci controlling ecologically and economically important traits.
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