Biolistic DNA delivery to leaf tissue of plants with the non-vacuum gene gun (HandyGun)

: Anssi L Vuorinen, Arto Nieminen, Victor Gaba, Sidona Sikorskaite, Jari P T Valkonen

: Sudowe S, Reske-Kunz AB

: 2013

Methods in Molecular Biology

: Biolistic DNA Delivery

: Methods in Molecular Biology

: 940

: 45

: 51

: 7

: 978-1-62703-109-7

: 978-1-62703-110-3

: 1064-3745

DOI: https://doi.org/10.1007/978-1-62703-110-3_4

: http://link.springer.com/protocol/10.1007/978-1-62703-110-3_4

Non-vacuum gene guns such as HandyGun are fl exible tools for bombardment of targets of varying size.
Construction of HandyGun is simpler and cheaper than vacuum gene guns and will be described here. The
conditions for maximal transient transformation ef fi ciency of plant cells with plasmid DNA using HandyGun
will be provided.
Non-vacuum gene guns such as HandyGun are fl exible tools for bombardment of targets of varying size. Construction of HandyGun is simpler and cheaper than vacuum gene guns and will be described here. The conditions for maximal transient transformation ef fi ciency of plant cells with plasmid DNA using HandyGun will be provided.