B2 Non-refereed book chapter or chapter in a compilation book
Biolistic DNA delivery to leaf tissue of plants with the non-vacuum gene gun (HandyGun)
Authors: Anssi L Vuorinen, Arto Nieminen, Victor Gaba, Sidona Sikorskaite, Jari P T Valkonen
Editors: Sudowe S, Reske-Kunz AB
Publication year: 2013
Journal: Methods in Molecular Biology
Book title : Biolistic DNA Delivery
Series title: Methods in Molecular Biology
Volume: 940
First page : 45
Last page: 51
Number of pages: 7
ISBN: 978-1-62703-109-7
eISBN: 978-1-62703-110-3
ISSN: 1064-3745
DOI: https://doi.org/10.1007/978-1-62703-110-3_4
Web address : http://link.springer.com/protocol/10.1007/978-1-62703-110-3_4
Abstract
Non-vacuum gene guns such as HandyGun are fl exible tools for bombardment of targets of varying size.
Construction of HandyGun is simpler and cheaper than vacuum gene guns and will be described here. The
conditions for maximal transient transformation ef fi ciency of plant cells with plasmid DNA using HandyGun
will be provided.
Non-vacuum gene guns such as HandyGun are fl exible tools for bombardment of targets of varying size. Construction of HandyGun is simpler and cheaper than vacuum gene guns and will be described here. The conditions for maximal transient transformation ef fi ciency of plant cells with plasmid DNA using HandyGun will be provided.
Non-vacuum gene guns such as HandyGun are fl exible tools for bombardment of targets of varying size.
Construction of HandyGun is simpler and cheaper than vacuum gene guns and will be described here. The
conditions for maximal transient transformation ef fi ciency of plant cells with plasmid DNA using HandyGun
will be provided.
Non-vacuum gene guns such as HandyGun are fl exible tools for bombardment of targets of varying size. Construction of HandyGun is simpler and cheaper than vacuum gene guns and will be described here. The conditions for maximal transient transformation ef fi ciency of plant cells with plasmid DNA using HandyGun will be provided.
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