Phosphodiester cleavage of diribonucleoside 3 ',3 '-monophosphates: Rate acceleration by the 2 '-hydroxyl and 2 '-amino function, implications to ribozyme action

: Ora M, Kiviniemi A, Lonnberg T, Virtanen S, Lonnberg H

: 2005

Collection Symposium Series

Chemistry of Nucleic Acid Components

: COLL SYMPOS SERIES

: 7

: 261

: 263

: 3

: 80-86241-25-4

The hydrolytic reactions of diribonucleoside 3',3'-monophosphates have been followed by RP HPLC over a wide pH-range at 363.2 K. The mechanisms of the reactions are discussed, particularly focusing on the possible stabilization of phosphorane intermediate and/or transition state via an intramolecular hydrogen bonding by the 2'-hydroxyl and 2'-amino group.

Chemical sciences