A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Sensitivity of Helicobacter pylori to an innate defence mechanism, the lactoperoxidase system, in buffer and in human whole saliva
Tekijät: Haukioja A, Ihalin R, Loimaranta V, Lenander M, Tenovuo J
Kustantaja: SOC GENERAL MICROBIOLOGY
Julkaisuvuosi: 2004
Journal: Journal of Medical Microbiology
Tietokannassa oleva lehden nimi: JOURNAL OF MEDICAL MICROBIOLOGY
Lehden akronyymi: J MED MICROBIOL
Vuosikerta: 53
Numero: 9
Aloitussivu: 855
Lopetussivu: 860
Sivujen määrä: 6
ISSN: 0022-2615
DOI: https://doi.org/10.1099/jmm.0.45548-0
Verkko-osoite: http://jmm.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.45548-0#tab2
Tiivistelmä
Helicobacter pylori has frequently been isolated from human dental plaque, and oral spread via saliva. is thought to be one of its principal modes of transmission. Among other innate defence systems human saliva contains peroxidase enzymes and lysozyme. The sensitivity of H. pylori to physiological concentrations of lactoperoxidase and its salivary substrate thiocyanate, and different amounts of hydrogen peroxide (H2O2) was investigated in buffer and in human whole saliva. The effect of lysozyme was also studied in saliva. All tested H. pylori strains, ATCC 43504(T) and five clinical isolates, were efficiently inhibited by the peroxidase system with high concentrations of H2O2 in buffer. The inhibition was stronger at lower pH. However, in human saliva these high concentrations of H2O2 generated less hypothiocyanite, the antibacterial product of the peroxidase system and the effects of the peroxidase system were weaker. Physiological concentration of lysozyme was not bacteriocidal against H. pylori, nor did it enhance the effect of the peroxidase system in saliva. Thus, further studies are needed to enhance the efficacy of peroxidase systems in human saliva to make it more beneficial not only against dental but also against gastric pathogens.
Helicobacter pylori has frequently been isolated from human dental plaque, and oral spread via saliva. is thought to be one of its principal modes of transmission. Among other innate defence systems human saliva contains peroxidase enzymes and lysozyme. The sensitivity of H. pylori to physiological concentrations of lactoperoxidase and its salivary substrate thiocyanate, and different amounts of hydrogen peroxide (H2O2) was investigated in buffer and in human whole saliva. The effect of lysozyme was also studied in saliva. All tested H. pylori strains, ATCC 43504(T) and five clinical isolates, were efficiently inhibited by the peroxidase system with high concentrations of H2O2 in buffer. The inhibition was stronger at lower pH. However, in human saliva these high concentrations of H2O2 generated less hypothiocyanite, the antibacterial product of the peroxidase system and the effects of the peroxidase system were weaker. Physiological concentration of lysozyme was not bacteriocidal against H. pylori, nor did it enhance the effect of the peroxidase system in saliva. Thus, further studies are needed to enhance the efficacy of peroxidase systems in human saliva to make it more beneficial not only against dental but also against gastric pathogens.
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