A1 Refereed original research article in a scientific journal
Sensitivity of Helicobacter pylori to an innate defence mechanism, the lactoperoxidase system, in buffer and in human whole saliva
Authors: Haukioja A, Ihalin R, Loimaranta V, Lenander M, Tenovuo J
Publisher: SOC GENERAL MICROBIOLOGY
Publication year: 2004
Journal: Journal of Medical Microbiology
Journal name in source: JOURNAL OF MEDICAL MICROBIOLOGY
Journal acronym: J MED MICROBIOL
Volume: 53
Issue: 9
First page : 855
Last page: 860
Number of pages: 6
ISSN: 0022-2615
DOI: https://doi.org/10.1099/jmm.0.45548-0
Web address : http://jmm.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.45548-0#tab2
Abstract
Helicobacter pylori has frequently been isolated from human dental plaque, and oral spread via saliva. is thought to be one of its principal modes of transmission. Among other innate defence systems human saliva contains peroxidase enzymes and lysozyme. The sensitivity of H. pylori to physiological concentrations of lactoperoxidase and its salivary substrate thiocyanate, and different amounts of hydrogen peroxide (H2O2) was investigated in buffer and in human whole saliva. The effect of lysozyme was also studied in saliva. All tested H. pylori strains, ATCC 43504(T) and five clinical isolates, were efficiently inhibited by the peroxidase system with high concentrations of H2O2 in buffer. The inhibition was stronger at lower pH. However, in human saliva these high concentrations of H2O2 generated less hypothiocyanite, the antibacterial product of the peroxidase system and the effects of the peroxidase system were weaker. Physiological concentration of lysozyme was not bacteriocidal against H. pylori, nor did it enhance the effect of the peroxidase system in saliva. Thus, further studies are needed to enhance the efficacy of peroxidase systems in human saliva to make it more beneficial not only against dental but also against gastric pathogens.
Helicobacter pylori has frequently been isolated from human dental plaque, and oral spread via saliva. is thought to be one of its principal modes of transmission. Among other innate defence systems human saliva contains peroxidase enzymes and lysozyme. The sensitivity of H. pylori to physiological concentrations of lactoperoxidase and its salivary substrate thiocyanate, and different amounts of hydrogen peroxide (H2O2) was investigated in buffer and in human whole saliva. The effect of lysozyme was also studied in saliva. All tested H. pylori strains, ATCC 43504(T) and five clinical isolates, were efficiently inhibited by the peroxidase system with high concentrations of H2O2 in buffer. The inhibition was stronger at lower pH. However, in human saliva these high concentrations of H2O2 generated less hypothiocyanite, the antibacterial product of the peroxidase system and the effects of the peroxidase system were weaker. Physiological concentration of lysozyme was not bacteriocidal against H. pylori, nor did it enhance the effect of the peroxidase system in saliva. Thus, further studies are needed to enhance the efficacy of peroxidase systems in human saliva to make it more beneficial not only against dental but also against gastric pathogens.
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