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Luminescence switching by hybridization-directed mixed lanthanide complex formation
Tekijät: Karhunen U, Jaakkola L, Wang Q, Lamminmaki U, Soukka T
Julkaisuvuosi: 2010
Journal: Analytical Chemistry
Tietokannassa oleva lehden nimi: Analytical Chemistry
Numero sarjassa: 2
Vuosikerta: 82
Numero: 2
Aloitussivu: 751
Lopetussivu: 754
Sivujen määrä: 4
ISSN: 0003-2700
DOI: https://doi.org/10.1021/ac9020825
Verkko-osoite: http://api.elsevier.com/content/abstract/scopus_id:75649110862
Tiivistelmä
We have developed a homogeneous assay method in which the lanthanide ion carrier and light absorbing components of a luminescent lanthanide chelate are separated in two distinct molecules that can together form a luminescent mixed chelate complex. The separated label moieties were conjugated to oligonucleotides which were used as probes to detect a complementary target DNA. The background signal of the assay was very low, indicating the signal was highly dependent on the hybridization of the two probes on adjacent positions on the target oligonucleotide. © 2010 American Chemical Society.
We have developed a homogeneous assay method in which the lanthanide ion carrier and light absorbing components of a luminescent lanthanide chelate are separated in two distinct molecules that can together form a luminescent mixed chelate complex. The separated label moieties were conjugated to oligonucleotides which were used as probes to detect a complementary target DNA. The background signal of the assay was very low, indicating the signal was highly dependent on the hybridization of the two probes on adjacent positions on the target oligonucleotide. © 2010 American Chemical Society.
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