A1 Refereed original research article in a scientific journal
Luminescence switching by hybridization-directed mixed lanthanide complex formation
Authors: Karhunen U, Jaakkola L, Wang Q, Lamminmaki U, Soukka T
Publication year: 2010
Journal: Analytical Chemistry
Journal name in source: Analytical Chemistry
Number in series: 2
Volume: 82
Issue: 2
First page : 751
Last page: 754
Number of pages: 4
ISSN: 0003-2700
DOI: https://doi.org/10.1021/ac9020825
Web address : http://api.elsevier.com/content/abstract/scopus_id:75649110862
Abstract
We have developed a homogeneous assay method in which the lanthanide ion carrier and light absorbing components of a luminescent lanthanide chelate are separated in two distinct molecules that can together form a luminescent mixed chelate complex. The separated label moieties were conjugated to oligonucleotides which were used as probes to detect a complementary target DNA. The background signal of the assay was very low, indicating the signal was highly dependent on the hybridization of the two probes on adjacent positions on the target oligonucleotide. © 2010 American Chemical Society.
We have developed a homogeneous assay method in which the lanthanide ion carrier and light absorbing components of a luminescent lanthanide chelate are separated in two distinct molecules that can together form a luminescent mixed chelate complex. The separated label moieties were conjugated to oligonucleotides which were used as probes to detect a complementary target DNA. The background signal of the assay was very low, indicating the signal was highly dependent on the hybridization of the two probes on adjacent positions on the target oligonucleotide. © 2010 American Chemical Society.
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