A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
HIC1 interacts with FOXP3 multi protein complex: Novel pleiotropic mechanisms to regulate human regulatory T cell differentiation and function
Tekijät: Andrabi SBA, Batkulwar K, Bhosale SD, Moulder R, Khan MH, Buchacher T, Khan MM, Arnkil I, Rasool O, Marson A, Kalim UU, Lahesmaa R
Julkaisuvuosi: 2023
Journal: Immunology Letters
Tietokannassa oleva lehden nimi: Immunology letters
Lehden akronyymi: Immunol Lett
Vuosikerta: 263
Aloitussivu: 123
Lopetussivu: 132
ISSN: 0165-2478
eISSN: 1879-0542
DOI: https://doi.org/10.1016/j.imlet.2023.09.001
Verkko-osoite: https://doi.org/10.1016/j.imlet.2023.09.001
Rinnakkaistallenteen osoite: https://research.utu.fi/converis/portal/detail/Publication/181811045
Transcriptional repressor, hypermethylated in cancer 1 (HIC1) participates in a range of important biological processes, such as tumor repression, immune suppression, embryonic development and epigenetic gene regulation. Further to these, we previously demonstrated that HIC1 provides a significant contribution to the function and development of regulatory T (Treg) cells. However, the mechanism by which it regulates these processes was not apparent. To address this question, we used affinity-purification mass spectrometry to characterize the HIC1 interactome in human Treg cells. Altogether 61 high-confidence interactors were identified, including IKZF3, which is a key transcription factor in the development of Treg cells. The biological processes associated with these interacting proteins include protein transport, mRNA processing, non-coding (ncRNA) transcription and RNA metabolism. The results revealed that HIC1 is part of a FOXP3-RUNX1-CBFB protein complex that regulates Treg signature genes thus improving our understanding of HIC1 function during early Treg cell differentiation.
Ladattava julkaisu This is an electronic reprint of the original article. |  |
