A1 Refereed original research article in a scientific journal
HIC1 interacts with FOXP3 multi protein complex: Novel pleiotropic mechanisms to regulate human regulatory T cell differentiation and function
Authors: Andrabi SBA, Batkulwar K, Bhosale SD, Moulder R, Khan MH, Buchacher T, Khan MM, Arnkil I, Rasool O, Marson A, Kalim UU, Lahesmaa R
Publication year: 2023
Journal: Immunology Letters
Journal name in source: Immunology letters
Journal acronym: Immunol Lett
Volume: 263
First page : 123
Last page: 132
ISSN: 0165-2478
eISSN: 1879-0542
DOI: https://doi.org/10.1016/j.imlet.2023.09.001
Web address : https://doi.org/10.1016/j.imlet.2023.09.001
Self-archived copy’s web address: https://research.utu.fi/converis/portal/detail/Publication/181811045
Transcriptional repressor, hypermethylated in cancer 1 (HIC1) participates in a range of important biological processes, such as tumor repression, immune suppression, embryonic development and epigenetic gene regulation. Further to these, we previously demonstrated that HIC1 provides a significant contribution to the function and development of regulatory T (Treg) cells. However, the mechanism by which it regulates these processes was not apparent. To address this question, we used affinity-purification mass spectrometry to characterize the HIC1 interactome in human Treg cells. Altogether 61 high-confidence interactors were identified, including IKZF3, which is a key transcription factor in the development of Treg cells. The biological processes associated with these interacting proteins include protein transport, mRNA processing, non-coding (ncRNA) transcription and RNA metabolism. The results revealed that HIC1 is part of a FOXP3-RUNX1-CBFB protein complex that regulates Treg signature genes thus improving our understanding of HIC1 function during early Treg cell differentiation.
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