A1 Refereed original research article in a scientific journal
Protocol for preparing dynamic covalent macrocycles for co-delivering genes and drugs to cancer cell lines
Authors: Lyu Yonglei, Wu Xiaoxia, Yang Jinghui, Wang Xin, Li Jianwei
Publisher: Cell Press
Publication year: 2023
Journal: STAR protocols
Journal name in source: STAR Protocols
Article number: 102350
Volume: 4
Issue: 3
ISSN: 2666-1667
DOI: https://doi.org/10.1016/j.xpro.2023.102350
Publication's open availability at the time of reporting: Open Access
Publication channel's open availability : Open Access publication channel
Web address : https://doi.org/10.1016/j.xpro.2023.102350
Self-archived copy’s web address: https://research.utu.fi/converis/portal/detail/Publication/180244591
Self-archived copy's licence: CC BY NC ND
Self-archived copy's version: Publisher`s PDF
Combination therapy using effective drug molecules and functional genes such as small interfering RNA (siRNA) has been suggested as a powerful strategy against multiple drug resistance. Here, we present a protocol for preparing a delivery system by developing dynamic covalent macrocycles using a dithiol monomer to co-deliver doxorubicin and siRNA. We describe steps for preparing the dithiol monomer, followed by co-delivery to form nanoparticles. We then detail procedures for cell uptake and assessing enhanced anti-cancer efficacy in vitro. For complete details on the use and execution of this protocol, please refer to Lyu et al.
Keywords:
material sciences
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