A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Protocol for preparing dynamic covalent macrocycles for co-delivering genes and drugs to cancer cell lines
Tekijät: Lyu Yonglei, Wu Xiaoxia, Yang Jinghui, Wang Xin, Li Jianwei
Kustantaja: Cell Press
Julkaisuvuosi: 2023
Lehti: STAR protocols
Tietokannassa oleva lehden nimi: STAR Protocols
Artikkelin numero: 102350
Vuosikerta: 4
Numero: 3
ISSN: 2666-1667
DOI: https://doi.org/10.1016/j.xpro.2023.102350
Julkaisun avoimuus kirjaamishetkellä: Avoimesti saatavilla
Julkaisukanavan avoimuus : Kokonaan avoin julkaisukanava
Verkko-osoite: https://doi.org/10.1016/j.xpro.2023.102350
Rinnakkaistallenteen osoite: https://research.utu.fi/converis/portal/detail/Publication/180244591
Rinnakkaistallenteen lisenssi: CC BY NC ND
Rinnakkaistallennetun julkaisun versio: Kustantajan versio
Combination therapy using effective drug molecules and functional genes such as small interfering RNA (siRNA) has been suggested as a powerful strategy against multiple drug resistance. Here, we present a protocol for preparing a delivery system by developing dynamic covalent macrocycles using a dithiol monomer to co-deliver doxorubicin and siRNA. We describe steps for preparing the dithiol monomer, followed by co-delivery to form nanoparticles. We then detail procedures for cell uptake and assessing enhanced anti-cancer efficacy in vitro. For complete details on the use and execution of this protocol, please refer to Lyu et al.
Avainsanat:
material sciences
Ladattava julkaisu This is an electronic reprint of the original article. |  |
