Assembly of split aptamers by dynamic pH-responsive covalent ligation



: Aho Aapo, Virta Pasi

Publisher: ROYAL SOC CHEMISTRY

: 2023

: Chemical Communications

: CHEMICAL COMMUNICATIONS

: CHEM COMMUN

: 59

: 38

: 5689

: 5692

: 4

: 1359-7345

DOI: https://doi.org/10.1039/d3cc01158e

: https://doi.org/10.1039/D3CC01158E

: https://research.utu.fi/converis/portal/detail/Publication/179466137


Reversible pH-responsive N-methoxyoxazolidine formation is used to ligate split aptamer fragments. Two twice-split models and one thrice-split model of CBA (cocaine-binding aptamer) were examined. The aptamer assembly was dynamic, proportional to the substrate concentration and occurred without interfering background ligation.

Last updated on 2024-26-11 at 13:26